Journal: Cell

Article Title: SINGLE-CELL CHROMATIN MODIFICATION PROFILING REVEALS INCREASED EPIGENETIC VARIATIONS WITH AGING

doi: 10.1016/j.cell.2018.03.079

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Biological Samples Buffy coat from whole blood (for bio rep 1 and 2) Stanford Blood Center https://bloodcenter.stanford.edu/research-labs/research-products-and-services/blood-products/ PBMCs from twin subjects Brodin et al. study (PMID: 25594173) DOI: http://dx.doi.org/10.1016/j.cell.2014.12.020 Chemicals, Peptides, and Recombinant Proteins Ficoll-Paque PLUS GE Healthcare 17-1440-02 RBC lysis buffer (10X) BioLegend 420301 DMSO Sigma D2650 TCEP ThermoFisher 77720 Sodium azide Sigma S2002 EDTA Fisher BP120-500 16% paraformaldehyde Electron Microscopy Sciences 15710 Methanol Fisher Scientific A454-4 PBS ThermoFisher 10010-072 Software and Algorithms FlowJo FlowJo, LLC https://www.flowjo.com/ Other SepMate-50 STEMCELL Technologies 85460 Human AB serum heat-inactivated Valley Biomedical HP1022HI Maxpar X8 multi-metal labeling kit Fluidigm 201300 Antibody stabilizer (PBS-based) Boca Scientific 131 000 RPMI 1640 media ThermoFisher 22400-105 Fetal bovine serum ATCC 30-2020 Bovine serum albumin Sigma A3059 Cisplatin ENZO Life Sciences ALX-400-040-M250 DNase/RNase-free distilled water ThermoFisher 10977-023 Cell-ID 20-Plex Pd Barcoding Fluidigm 201060 Human TruStain FcX for Fc receptor blocking BioLegend 422302 Cell-I Intercalator-Ir—500 μM Fluidigm 201192B EQ four element calibration beads Fluidigm 201078 Zombie aqua fixable viability kit BioLegend 423102 Open in a separate window KEY RESOURCES TABLE Contact for Reagent and Resource Sharing Further information and requests for resources and reagents should be directed to, and will be fulfilled, by the Lead Contact, Alex J. Kuo ( ude.drofnats@9220xela ).

Techniques: Formulation, Recombinant, Red Blood Cell Lysis, Electron Microscopy, Software, Labeling, Blocking Assay